Chromogenic & Fluorogenic Substrates

Specific synthetic peptide substrates for the measurement of the activity of various serine proteases in the field of coagulation, fibrinolysis, kallikrein-kinin system, complement system, tissue degradation, angiogenesis, cancer and inflammation.

These peptides substrates can also be used in the quality control of pharmaceutical and other preparations.

 


Chromogenic Substrates for Factor Xa 

Name Formula Package  Reference
Chrom-65 D-Arg-Gly-Arg-pNA  25 mg  5D-8527-25MG

Molecular weight : 714.6 Da

Km (mM): 0,26

kcat (1/s): 240

Chrom-65 is identical to reference HS0171 which comes with instructions for use for heparin testing according to the EP and USP

Name Formula Package  Reference
Chrom-32 Suc-Ile-Glu (ϒ Pip)Gly-Arg-pNa, HCl  25 mg  5D-8020-25MG

Molecular weight : 760.9 Da

Km (mM): 1,5

kcat (1/s): 230

Name Formula Package  Reference
Chrom-88 CH3OCO-D-CHA-Gly-Arg-pNA 100 μmol  5D-8506-100UM

Molecular weight :622.7 Da

Km (mM): 0,106
kcat (1/s): 140

 

Name Formula Package  Reference
Chrom-22  Bz-Ile-Glu (OCH3)-Gly-Arg-pNa
and Bz-Ile- Glu (OH)Gly-Arg-pNa
25 mg  5D-8822-25MG

Molecular weight : 711.8 Da ( 1) and 697.7 Da ( 2)

Km (mM): 1,1

kcat (1/s): 100


Chromogenic Substrates for Thrombin

Name Formula Package  Reference
Chrom-38 H-D-Phe-Pip-Arg-pNa 25 mg  5D-8166-25MG

Molecular weight : 625,6 Da

Km (mM): 0,0070

kcat (1/s): 180

Chrom-38 is identical to reference HS0170 which comes with instructions for use for heparin testing according to the EP and USP

Name Formula Package  Reference
Chrom-81 Tos-Gly-Pro-Arg-pNa. AcOH 25 mg  5D-8881-25MG

Molecular weight : 662.6 Da


Chromogenic Substrate for tPA and Broad Spectrum 

Name Formula Package  Reference
Chrom-tPA H-D-Ile–Pro-Arg-pNa-2HCl 25 mg  5D-9101-25MG

Molecular weight : 504.6 Da

Km (mM): 1,0 (for single chain tPA)

kcat (1/s): 10 (for single chain tPA)


Fluorogenic Substrate for Factor IXa 

Name Formula Package  Reference
Fluor IXa CH3SO2-D-CHA-Gly-Arg-AMC⋅AcOH 50 μmol  5D-9503-50UM
10 μmol  5D-9503-10UM

The fluorogenic group for these substrates is AMC (7-Amino-4-methylcoumarin) with and absorption maximum at 342 nm and emission at 440 nm.

Molecular weight :679.8  Da

Km (mM): 0,22

kcat (1/s): 163


Chromogenic Substrate for Activated Protein C

Name Formula Package  Reference
Chrom-66 p-Glu-Pro-Arg-pNa. HCl 25 mg  5D-8866-25MG

Molecular weight :  502.5 Da

Km (mM): 0,20

kcat (1/s): 190


 

Chromogenic Substrate for Kallicrein

Name Formula Package  Reference
Chrom-02 D-Pr0-Phe-Arg-pNa.2HCl 25 mg  5D-8802-25MG

Molecular weight :  538,6 Da

Km (mM): 0,22

kcat (1/s): 150


Chromogenic Substrate for Plasmin

Name Formula Package  Reference
Chrom-03 PyroGlu–Phe-Lys-pNa-HCl 25 mg  5D-8803-25MG

Molecular weight : 524,6 Da

Km (mM): 0,20

kcat (1/s): 190


Chromogenic Substrate for Urokinase

Name Formula Package  Reference
Chrom-44 pyro-Glu-Gly-Arg-pNa.HCl 25 mg  5D-8008-25MG

Molecular weight :  462,5 Da

Km (mM): 0,08

kcat (1/s): 11


Chromogenic Substrate for Factor XIa

Name Formula Package  Reference
Chrom-66 p-Glu-Pro-Arg-pNa. HCl 25 mg  5D-8866-25MG

Molecular weight :  502.5 Da

Km (mM): 1,2

kcat (1/s): 340


*kinetic data mentioned on this page were obtained under strictly standardised conditions with  human enzymes