Thrombodynamics: Time- and Space-resolved imaging of coagulation

Real-time visualisation of clot propagation

Observation of spontaneous clots formation

Now introducing:  Thrombodynamics T2-T = Thrombodynamics T2-F for Fibrin registration  + Thrombin Generation

Thrombodynamics is based on spatial separation of activation and propagation phases of coagulation.

The coagulation is activated by a special surface with immobilized tissue factor (TF), which reconstructs damage vessel wall. As soon as blood plasma sample comes into a contact with TF the coagulation process initiates and fibrin cot starts growing in a thin layer of non-stirred plasma, placed into a plastic cuvette.

Clot formation starts on the surface of the activator and propagates into the bulk of plasma. Images of clot formation are registered via CCD camera using a time laps microscopy mode in scattered light.

 

Parameters
Tlag, [min] – Lag time – time between contact of plasma sample with activator and start of clot growth. Tlag characterizes the initiation phase of blood coagulation.
V, [µm/min] – Average rate of clot growth. The parameter characterizes the propagation phase of blood coagulation.
Tsp, [min] – Tsp is the time of spontaneous clots formation in a plasma sample volume, which had no initial contact with activating insert. Spontaneous clotting is induced by circulating activators, active coagulation factors and microparticles.
Complementary Parameters
Vi, [µm/min] – Initial rate, it characterizes the initiation phase of clot growth.
D, [a.u.] – Clot density, it is an optical parameter, which is equal to intensity of light scattering from a fibrin clot.
CS, [µm] – Clot size on the 30th min of the measurement.